The relevance of our research work is due to the fact that in the process of hepatocyte cultivation, it is necessary to test drugs and propose an optimal approach to treatment, using it to determine the possibility of treating hereditary or terminal liver diseases by transplantation. The characteristics of the growth dynamics of primary cultured hepatocytes in a standard nutrient medium with flow cytofluorimetry, light microscope, electron microscope and immunofluorescence analysis methods are given. It was found that the growth of hepatocytes stops after 24 hours of the cell cycle after the G0/G1 stages and that they survive for 48 hours of the experiment without increasing the percentage of cells at the apoptosis stage. A decrease in the absolute volume of cells and an increase in the nuclear–cytoplasmic ratio were revealed, which indicates a decrease in the proportion of cytoplasm in hepatocytes during growth. A decrease in the volume density of glycogen and mitochondria and the growth of basal autophagy in the cytoplasm of cells – the predominance of glycogenophagy and mitophagy – were shown. Thus, autophagy is a mechanism that provides cellular homeostasis of isolated hepatocytes.
Objective: to identify basal autophagy in the cytoplasm of isolated hepatocytes in the dynamics of their cultivation.